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M9 is a chemical defined minimal medium supporting the growth of "wild-type" strains of E
2 Glucose 2 KH2PO4 1
MOPS is an excellent buffer for many biological systems at near-neutral pH
Tryptone 35 Yeast Extract 20 NaCl 5 Agar 15 Final pH 7
15 Suspend 55
LB-Top Agar Miller Size:250g M9 is a chemical definedFormula g l Tryptone 10 Yeast extract 5 NaCl 10 Agar 7 Final pH 7. 0 0. 2 at 25C Suspend 32 gram powdered medium in 1 litre distilled water Store dry at room temperature
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