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15 (NH4)6M07O24
Assay (HPLC) ≥99% Melting range 148
resulting in an increase of cell density up to 5 x 10^7
Tryptone 35 Yeast Extract 20 NaCl 5 Agar 7 Final pH 7
2 at 25°C Suspend 30 gram powdered medium in 1 litre distilled water
SIH Medium Size:250g 15 (NH4)6M07O24SIH medium, developed by Hanh, Friehs and Flaschel (2004), is the next step in the development of synthetic media designed to grow D. discoideum in high cell densities. SIH medium is an improved version of FM medium, as developed by Frank and Kessin (1977). The main difference between SIH and FM is the novel addition of Aspartic acid (1. 1 mM) and increased levels of Lysine (8. 5 mM) and Tryptophane (1. 7 mM). Concentrations of Cysteine, Glutamic